Evaluation of Aneurinibacillus aneurinilyticus isolated from corn steep liquor as source of Biosurfactants, Gramicidin S and Polyhydroxyalkanoates

Paper ID: 
cest2025_00060
Topic: 
2. CIRCULAR ECONOMY AND BIOECONOMY
Published under CEST2025
Proceedings ISBN:
Proceedings ISSN: 2944-9820
Authors: 
(Corresponding) Moldes A., Lvova K., González-Fernández N., López-Prieto A., Vecino X., Pérez-Cid B., Cruz J.
Abstract: 
This study investigates the potential of Aneurinibacillus aneurinilyticus (A. aneurinilyticus), isolated from corn steep liquor, as a sustainable source of valuable bioproducts such us biosurfactants, Gramicidin S homologs, and polyhydroxyalkanoates (PHAs). Using phosphate-buffered saline (PBS), a biosurfactant extract was successfully extracted from A. aneurinilyticus cells, demonstrating its surface-active properties. In addition, ethanol was employed to extract Gramicidin S homologs from A. aneurinilycus cells, showcasing their potential to obtain new antibiotics against Gram-positive bacteria to address drug-resistant pathogens. Finally, vacuoles in A. aneurinilyticus were identified as potential reservoirs for PHAs, biopolyesters that can serve as sustainable alternatives to petroleum-based plastics.
Keywords: 
Circular economy, antibiotic, biosurfactant, bioplastic, Bacillus, sustainability