Screening thousands of emerging contaminants in Asopos river basin by UPLC-QToF-MS

Monitoring activities over several decades have revealed the ubiquitous presence of organic contaminants in environmental compartments. Asopos river basin, located in Sterea Ellada, has been the focus of many environmental studies over the last decades, mainly due to its geomorphology and the extensive industrial activities that take place in this territory. The detection of high Cr(VI) concentrations, causing potential carcinogenic effects, have attracted the attention of media and raised the consciousness of the citizens. Although there are many studies in recent literature focusing on the occurrence of heavy metals in Asopos river basin, there is a lack of information concerning the presence of emerging contaminants. The aim of this study is the determination of thousands of emerging contaminants in the environmental samples (river water and sediments) form Asopos river basin, following wide-scope target screening methodologies. Solid-Phase Extraction (SPE) by mixed-mode sorbent was used during the sample preparation of river water samples to ensure the extraction of various classes of compounds with a wide range of physicochemical properties. The extracts were analyzed by complimentary chromatographic techniques, including Reversed Phase (RP) and Hydrophilic Interaction Liquid Chromatography (HILIC) coupled to Quadrupole-Time-of-Flight Mass Spectrometry (QToF-MS). Moreover, the application of suspect and non-target screening, using advanced chemometric techniques, will provide a holistic view of the pollution in the area under investigation.