Biological activity of flufenamic acid and synthesized derivates (PFCs) that may occur in the environment

Polyfluorinated compounds (PFCs) are used in the field of pharmaceuticals, pesticides, impregnating agents, etc. These are predominantly compounds that are difficult to degradate in the environment, and therefore are highly persistent. Many these compounds are known to have toxic effects and thus a negative effect on humans and the entire ecosystem. PFCs include, for example, flufenamic acid (antipyretic). The aim of the study is to verify the biological activity of flufenamic acid and derivatives prepared from this initial compound by organic synthesis. Flufenamic acid is a known substance, however the prepared derivatives are chemical compounds that have not been described and characterized in detail. In the synthesis of prepared compounds, whose structure was confirmed by NMR spectroscopy, antimicrobial potential was tested against a wide range of microorganisms by the disc diffusion method and microdilution method in microtiter plates. Some antimicrobial properties of some of the prepared derivatives have been found to be close to the antimicrobial effect of the previously described and known antibiotic agents. These are significant and extremely stable pollutants in the environment, which, however, may have interesting antimicrobial properties according to the obtained results.