COMPLEX APPROACH FOR ANALYSIS OF CHANGES IN SOIL MICROBIOME

In the few past decades, genetic approaches for analysis of soil microbiome, e.g pyrosequencing, gained major attention and cultivation techniques were pushed into the background. The combination of several techniques could be useful and cheap approach for analysis of changes in soil microbiome. In our study of effect of silver nanoparticles on soil microbiome we exploited classical microbiological and biochemical methods for assessment of microbial structure. Effect caused by addition of silver nanoparticles was concentration dependent, the highest concentration of silver nanoparticles caused, besides the others, significant decrease in dehydrogenase activity of soil microorganisms. Concentration dependent changes in soil microbiome were also detected, e.g. in total numbers of cultivable microorganisms, cellulolytic or peroxidase. CLPP indicated significant shift in microbial diversity in all levels of substrate utilization.